Open hours for November 18-19, 2014.

Lab Materials

Lab Materials

Information to include in the Materials and Methods section.  Rewrite this information in your style.

Equipment and Software

MicroAquarium™: is a 75 X 50 X 4mm glass chamber designed for studying small aquatic organisms with a compound or dissecting microscope.  It is made with two 75 X 50 cm microscope slide glasses sandwiched together with a 2mm space between.  It is sealed on three sides (two short and one long side) with 100% Silicone Aquarium Sealant from All-Glass Aquarium Co., Franklin, WI– www.all-glass.com. The MicroAquarium™ is stored vertically (open end up) in a plastic stand with a clear plastic cap over the opening.

Microscopes: Non – camera compound microscopes: Observations of MicroAquarium™ made using Micromaster by Fisher Scientific compound microscope at 40X and 100X objectives.  http://www.olympusamerica.com/

Microscopes:

Compound microscopes with digital cameras: Olympus CH30 (http://www.olympusamerica.com/) or Leitz Laborlux 11, Leitz Laborlux D or Leitz Wetzlar (http://www.leica-microsystems.com ) with trinocular heads.  Images made at 40X, 100X and 400X (long focus objective).

Dissecting Microscope: Olympus S240 with 0.67- 4.0 zoom objective and trinocular head (http://www.olympusamerica.com/).

Cameras:

Compound Microscopes: Sony Handycam HDR-HC9, HDR-XR500v (http://www.sony.com/index.php), or Canon EOS T3i cameras for digital imaging and video recording. Cameras mounted to the trinocular head on the Olympus Ch30 and Leitz compound microscopes with to a Martin Microscope MM99 Adapter S/N: 4310 (http://www.martinmicroscope.com/) adapter.

Dissecting Microscope: Infinity 2 – Lumenera Corp www.Lumenera.com  7 Capella Court. Ottawa, On K2E 8A7 Canada

Computers: Dell Inc. (http://www.dell.com/) Optiplex 755 or Optiplex 980. Operating system Microsoft Windows XP.

Software: Images processed with Adobe Photoshop Elements 7.0. Adobe Systems Incorporated, 345 Park Ave, San Jose CA, 95110. Videos processed with Pinnacle Studio 12.1. Pinnacle Systems, Inc, 280 North Bernardo Ave Mountain View, CA 94943.

Lab room 507 Hesler environment from October  21 – November 13, 2014

Temperature: range 20.6⁰ – 22.7⁰ C

Light sources: primary lighting provided from 2 – 1.2 m long 34 watt cool light fluorescent lamps suspended 70 cm above MicroAquarium™.  Incidental lighting from classroom’s 1.2 m long 40 watt cool light fluorescent ceiling fixtures.

Light energy: 6 -10 PAR^* (light energy range – low value from suspended light fixture, high values with suspended light fixture and overhead room ceiling lights)

Light cycle: suspended light fixture illumination 24 hrs/day and ceiling lights illumination at random time periods between 8:00 AM – 12:00PM during duration of experiment.

^* A measure of photosynthetic active radiation, between 400 to 700 nanometers, photosynthetic organisms absorb to drive the light reaction of photosynthesis.

HOW TO CITE THIS BLOG

HOW TO CITE THIS BLOG

Bibliography.

McFarland, Kenneth [Internet] Botany 111 Fall 2014. [cited ADD DATE]. Available from http://botany1112014.blogspot.com/

In text citation.

 (McFarland, 2014)

An inquiry into the dynamic microorganisms in our environment.

The term project is adjunct to normal course work and is worth 85 points of your grade.

 

OBJECTIVE
The living organisms in our world are varied in life form, complexity and diversity.  During the course of the semester we will be looking at 4 of the 5 Kingdoms of living organism, some on a micro scale and others on a macro scale.  Our systematic overview of these organisms gives you a limited general perspective of the diversity of life.  This project will allow you to observe many of the smaller organisms seen in lab in a dynamic environment.

BACKGROUND

Life on earth is diverse and ubiquitous, filling large and small niches but limited by temperature, water and energy resources.  Individual organisms live in a community where they are competing for space and resources.  The complexity of these communities can vary depending on where they are located.  Warm wet environments generally have greater species diversity than cold and/or dry environments.  Within a defined environment, organisms vary greatly in size.  Because of the limitation of the human eye’s perception, we divide the organisms into macro and micro categories. 
After a rain, the pool of water which forms on the previous dry sidewalk will in a short time flourish with many tiny organisms.  The bark on a tree harbors many microorganisms which are usually dormant when the bark is dry.  When wetted these organisms come to life but are only visible with the microscope aided eye.  In ponds organism are stratified at different levels in the water – all occupying different niches. You can go anywhere in the environment, collect organic material, give it moisture and nutrients and watch the many small organisms emerge and compete among themselves for resources.

Procedure

Each student will have their own MicroAquarium™ to study a collection of microorganisms.  During lab (posted in syllabus) you will be setting up your individual MicroAquarium™.  The MicroAquarium™ will be kept in Hesler Biology Building room 507– three floors above the 204 lab room.
Setting up your MicroAquarium™

In the lab will be an assortment of waters collected from various sources which can be used in your MicroAquarium™.  You can also collect your own material and extract the organisms for your MicroAquarium™.  Let your instructor know if you want to do the later.  Record the source of material used in your aquarium.

1.      Obtain a MicroAquarium™.  It will have a glass tank, a stand holder and lid.

2.      Using the color dots provided, code your tank as follows.

a.       On the left hand side edge, place three colored dots in a vertical column as follows

                                                              i.      The top dot will be the color designated for your lab section.

                                                            ii.      The second dot will indicate the table you sit at during lab.

                                                          iii.      The third dot will be the seat number at your table.

b.      Now write your initials on the three colored dots.

3.      Using a pipet, extract water from one of the containers on the lab bench or from water sources in the greenhouse or from the extraction you have created.

a.       Extract enough water from the bottom of the container to fill the MicroAquarium™ tank about 1/3 full.

b.      Extract the next 1/3 of water for your tank from the middle layer.

c.       Then fill the rest of your tank with water from the surface.

4.      Place your tank in its stand, then decorate it with some plant parts or mosses or other objects provided.

Observing the contents of your MicroAquarium™.

You can observe the contents of the MicroAquarium™- with a hand lens or microscope. 

1.      Using a hand lens.

a.       Hold the hand lens in one hand and bring the lens up close to your eye.

b.      With the other hand hold the tank, without tipping the open end, and bring it up to toward the hand lens.  You will focus your view by moving the tank back and forth and not the hand lens.

2.      Using the microscope.

a.       Remove the stand and lid from the MicroAquarium™ tank.

b.      Gentle lay the tank on the microscope stage with the open end away from you.  The water will not come out. When using the compound scope you can place it in the slide holder and move the tank with the appropriate knobs.  On the dissecting scope lay the tank flat and manipulate it with your fingers.

Things to look for and record.

The tank with have living organisms and various forms of debris.

1.       Stationary vs. moving organisms.

2.       If moving, a description of motion.

3.       Single celled vs. multicellular organisms

4.       Chlorophyll green vs. non-green organisms.

5.       Habitat preference of the different organisms – bottom, middle or tops

6.       For each organism record if you have seen only one or many of them.

Observing your MicroAquarium™ each week.

You are to come and observe your MicroAquarium™ at least once a week over the next four weeks.

BRING YOUR NOTES AND OBSERVATIONS FROM EACH WEEK

The MicroAquarium™ and other equipment will be located in Hesler Biology Building  room 202– two doors from the Botany 111 lab in room 204. With the assistance your instructors you will be identifying a few of the organisms in your tank and make some of the following observations.

1.      What changes have occurred from the last observation?

2.      Have the numbers of each organism increased, decreased or stayed about the same?

3.      Are there dead organisms in the bottom layer of the tank?

4.      Create a list of the organism you observed and a book citation for its identification.

Open times for you to come and view your MicroAquarium™ will be posted by the door of Room 202  An instructor to assist you in your observation will be present during these times.

YOUR BLOG

A community project:  In order for all the students and teaching staff in the class to interact on this project, each student will record their project in a personal blog.  All blogs will be indexed in a Class blog: http://botany1112014.blogspot.com/   The class blog will provide you with information on the project as time goes on.  It will also have a listing of all the other student blogs by lab sections.  Your blog will be listed by your Display name, Blog title or created pseudonym which ever you prefer.  We will not use your real name in the index. 
How to set up a blog:  Set up your blog at “Blogger.com” through Google.

1.      Go to https://www.blogger.com/

2.      Click on the orange Create a Blog button and follow instructions.

Then send the following information to Botanylab@gmail.com

1. Your name and lab section  - this information will NOT be displayed in the class blog index – only your lab instructor will have access to this information

2. Your Display name – the name you want displayed in the class Blog to identify you

3. Blog title

4. Blog address URL which will look like http://YOURBLOGNAME.blogspot.com

What goes in your blog?

Your blog is an open notebook of your activities while working on this project.  You should add to your blog each week – each week’s entry should appear before Monday of the following week.

1.      Things you should include

a.       The name of the water source you used in setting up your MicroAquarium™ including other objects you might have added.

b.      Observations you make each week from your MicroAquarium™.

c.       Images of organisms:  A minimum of five images/videos are to be included. All images should be yours.  NO IMAGES EXTRACTED FORM THE WEB OR COPIED FROM OTHER SOURCES.  FOR EACH IMAGE YOU CREATE SITE A BOOK SOURCE FOR THE IDENTIFICATION OF THE ORGANISMS. 

2.      When searching for information on the web include URL’s you found useful and what information was pertinent to your project. Use the CBE style guide Appendix E on page 165 of the lab book.

a.       A list of the organisms found in your MicroAquarium™, approximate numbers observed, date when first observed and date when last observed.

b.      Image of the organism in your MicroAquarium™ - image equipment is available in room 202.

3.      Other pertinent comments, questions, or images you want to add are up to you. 

4.      Since this information can be read by others make sure you compose complete sentences with correct spelling and grammar.

Term Project report Due 5:00 PM Nov. 26,  2014: 30 PTS.

Submit printed and digital copy of your written term project report to your laboratory instructor.  No term project reports will be accepted after 5:00 PM Nov 26 2014.  

What to include in your Lab report.

Document Format

·         Typed – text double spaced.  Use 10 or 12-point font including tables and graphs.

·         Each section heading in bolded.

·         Tables, graphs and figures are numbered and include a title i.e. (Table 1, Comparison of aerobic and anaerobic respiration)

·         Follow the CBE style guide appendix E of lab book.  Understand the difference between “in text citations” and “bibliography”.

Format for Reporting MicroAquarium™ Project Research Results

Your Blog and this report are two separate instruments of communication.  DO NOT CUT AND PASTE YOUR BLOG INFORMATION OR IMAGES INTO THE LAB COMMUNICATION. The Lab Communication is an explanation of what you did.  Your reader can go to your blog to see your observations.

 

TITLE - Title for your Microaquarium project.  It should be succinct in describing your investigation. 

A.    Under title give your 1) Name, 2) Section number 3) “Display Name” (as used in the class blog) and 4) blog URL.

PART I  – Introduction (5 points) 
 

A.    What was the scope of this investigation?

B.     Give a description of each kingdom of organism that applies to your study –- Eubacteria, Protista, Plantae, Fungi, and/or Animalia   

C.    Discuss the ecosystem these organism live in and how it might affect their life cycle, diet, and behavior? 

D.    What water source did you use? What do you know about it? 

E.     Describe the utility of the MicroAquarium™ in your investigation of the above organisms?

PART II – Materials and Methods   (5 points) What did you do?

A.    How did you setup your MicroAquarium™? Give a complete description of how the MicroAquarium™ was assembled and material placed inside it. Be explicit and descriptive!

B.      Describe the equipment used and how it was used in making observations.(See Class Blog for equipment details)

C.    Describe the environmental conditions the organisms lived in while in the lab room and what and when things were added (See Class Blog for environment details of lab room.)

D.    What kind of observations were you recording in your blog? Explain what you were recording and not specific events you recorded.

E.     How many observations did you make and the time intervals between?

PART III – Results  (5 points) What did you find?

A.    Using the table format below list the organisms (including the plants you added) found in your MicroAquarium™

Create a table with these column heading: Name of Organism; Eubacteria; Single Celled Protista; Muticellular Protista; Fungi; Plantae; Animalia; during which observation (1, 2, 3, 4, 5) did they first appear and relative abundance( 1, 2-10, 11 – 20, 21-100^rd ).  List all your organisms (scientific and common name) in the first column then indicate with a “X” which kingdom they belong in.

Table 1: Describe the information this table provides for the reader

 

Organism	Eukaryote Or Prokaryote	Eubacteria	Single Celled Protista	Multicellular Protista	Fungi	Plantae	Animalia	Observation When First Appeared	Relative Abundance
Rana catesbeiana Bullfrog 1	E						X	5	1
Next organism

Provide in text citation below the table as shown and the information source for each citation in Part V.
¹  Kroug, D. 2002
²  Next publication source
 

PART IV – Summary and Discussion (10 points)

A.    SUMMARIZE the events that occurred in your MicroAquarium™. Discuss the impact the Betta food pellet and any other events (like a broken glass event) had on your MicroAquarium™.  DO NOT COPY AND PASTE YOUR OBSERVATIONS and images FROM YOUR BLOG

B.     From the class blog - find TWO other people who used the same water source (define the common water source) as you and compare their findings to yours.  Cite these sources as a web site.

C.    Similarly select TWO other people who had different water sources (define their water sources) than you and compare their results to yours. Cite these sources as a web site.

Part V - Bibliography (5 Points) What references did I use for this report? DO NOT COPY AND PASTE YOUR BIBLIOGRAPHY FROM YOUR BLOG.  If you used the same resources you can include them here.

Accurately cite the information sources you used. Use the CBE style guide Appendix E on page 165 of the lab book or http://www.lib.washington.edu/help/guides/42cbe.pdf  as a guide.  

This covers standard format styles for different types of information sources ie books, journals, internet etc.

To keep track of all your citations use Endnotes at http://libguides.utk.edu/endnote see tutorials on this page on getting started.  When using information you have acquired from other sources cite the source at the end of the sentence it was used in and not at the end of paragraph.

What do I turn into my lab instructor before 5:00 PM November 26, 2014?

·         A printed and digital copy of your lab report.

·         Include your:

o    1) Name,

o   2) Section number,

o   3) display name as posted in class blog

o   4) blog URL 

Grade Structure for Term project.

1.  Blog setup by due date                                                                                                  5 pts

2.  Blog entries observations:  initial setup and next four weeks -10 pts each                 50 pts
3.  Lab report                                                                                                                     30 pts
4.  Total points  
                                                                                                                 85 pts

Web sites of interest.

 Home of the MicroAquarium™  http://www.buildingthepride.com/faculty/microaquarium/index.htm

Microscope: http://starcentral.mbl.edu/microscope/portal.php
 

Micrographia: http://www.micrographia.com/index.htm
 

Pond life Identification kit: http://www.microscopy-uk.org.uk/index.html?http://www.microscopy-uk.org.uk/pond/index.html
 

The Smallest Page on the Web: http://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/wimsmall/smal1.html
 

Nikon Pond Life Video Gallery http://www.microscopyu.com/moviegallery/pondscum/index.html